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Quantification of lipogenesis gene expression To quantify gene expression, we used the Pure Link RNA Mini kit (12183025, Invitrogen, USA), Hilden, Germany) to extract total RNA from untreated and treated HepG2 cells and used High-Capacity cDNA Reverse Transcription kit (4368813, Applied Biosystems, Foster City, CA, USA) and 2 g total RNA to prepare cDNA

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A single failed study requiring repeat dosing and extended observation periods typically costs 510 times the value of a discarded vial

Novo Nordisks Peter Johannsen, MD, highlighted the biological signals observed and acknowledged the clinical limitations: subgroup analyses did not reveal meaningful benefit, and any potential responder group would be very small