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Note that due to the relative small size of the rats, rat fistulas regularly appeared as large and complex, and thereby, in this respect, corresponded to the worst presentation in the patients (Skorjanec et al., 2009

Cells were then resuspended in 500 L of free HBSS, strained through a 40 m cell strainer (BD Falcon), and analyzed using a flow cytometer (BD LSRFortessa Cell Analyzer, BD Biosciences) equipped with 488 nm laser for excitation

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