Flow cytometry and sorting For surface staining cells were incubated at 48 C, in the dark with fluorochrome-conjugated CD19, CD23, CD21-CD35 or CD35, IgD, IgM, CD1d, CD24, CD93, CD95, and GL-7 diluted 1:200 (refer to Supplementary Data 4 for details)
Inflammasome activation, macrophage infiltration, and cytokine production have been documented in cystinosis, indicating that chronic inflammation plays a key role in disease pathogenesis [147]
Neisseria meningitidis tonB, exbB, and exbD genes: Ton-dependent utilization of protein-bound iron in Neisseriae
Further characterization of metabolite compartmentalization and associated enzymatic activity revealed that blood NADs and glutathione predominantly reside within cellular fractions, with plasma containing only trace amounts of NADH and no detectable glutathione (Fig