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Afterwards, the final volume was adjusted with extraction buffer (50 mM Tris-HCl with 1.0 mM EDTA, pH 7.4), and the absorbance was measured at 412 nm with a dual-beam scanning spectrophotometer (AJX-6100PC, Micronal, Sao Paulo, Brazil) ( 2.13 Malondialdehyde content A total of 450 g of tissue homogenate protein was combined with 200 L of 30% (v/v) trichloroacetic acid (TCA)

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