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The pellets remaining after the second centrifugation were washed with buffer-1 for 15 min and centrifuged at 15,000 g for 10 min, and the resulting pellets were incubated with buffer-2 [20 mM HEPES/KOH (pH 7.8), 1.5 mM MgCl 2 , 420 mM NaCl, 0.2 mM EDTA, 25% glycerol, 0.5 mM dithiothreitol, 0.2 mM phenylmethylsulfonyl fluoride, and 1 mM Na 3 VO 4 ] for 20 min

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It's also the most scrutinized anemia code in claim submissions, and defaulting to it when an iron deficiency anemia icd 10 option or another specific code fits the documentation creates preventable denials

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