The bound antibodies were detected with secondary antibodies conjugated with IRDye680 or IRDye800 and analysed with an Odyssey Imager system (LI-COR
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( L , M ) 67NR cells over-expressing both FADS1 and FADS2, FADS1/2 OE , ( L ) were grown for 24 h in the presence of increasing doses of RSL3 and erastin ( M ) before assaying cell viability ( n = 3 biological replicates)
Disclosure of interest: Nothing to disclose E-Posters - ACUTE ISCHEMIC STROKE MANAGEMENT 01.00 - ACUTE ISCHEMIC STROKE MANAGEMENT - 01.02 - ACUTE MANAGEMENT - Thrombolysis or thrombectomy P2078 - ESOC25-1486 OUTCOMES AFTER REPERFUSION THERAPIES IN PATIENTS WITH TANDEM OCCLUSIONS Felicia Glavan 1,2 , Natalia Ciobanu 1,2 , Danu Glavan 1,2 , Andrei Filioglo 1 , Diana Manea *1 , Roman Smolnitchi 1 , Stanislav Groppa 1,2 1 Institute of Emergency Medicine, Chiinu, Moldova, 2 Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinu, Moldova Background and Aims: Tandem occlusions (TO) involving the extracranial internal carotid artery (ICA) and various intracranial arteries account for 13-30% of all large vessel occlusions (LVO)