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Although rare, serious side effects may occur including metabolic acidosis, kidney stones, decreased sweating leading to hyperthermia, and acute angle-closure glaucoma

The reaction conditions consisted of an initial denaturation step at 95 C for 5 min, followed by 40 cycles of denaturation at 95 C for 30 s, annealing at 57 C for 30 s, and extension at 72 C for 30 s

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