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Critically for lipid metabolism, SAMe is required for the synthesis of phosphatidylcholine (PC) via the phosphatidylethanolamine N-methyltransferase (PEMT) pathway

The tissues were counterstained with DAPI (4,6-diamidino-2-phenylindole) for 10 min at room temperature to label the nuclei and then mounted on slides with anti-fade medium (ProLong Gold, Thermo Fisher Scientific Inc., Rockford, IL)

These are exciting prospects that could revolutionize how we approach metabolic studies

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