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Punches of the following brain areas were extracted: lateral septum (LS), arcuate nucleus, dorsomedial and dorsolateral nucleus of the hypothalamus (HYP), periventricular nucleus of the hypothalamus (PVN), pontine gray (PG), parabrachial nucleus (PBN), and the nucleus of the solitary tract and area postrema (NTS/AP)

Dosing schedule and titration protocol Semaglutide titration follows a stepped approach designed to minimize gastrointestinal side effects