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Superposition of the third trajectory middle stage with the previous active GLP-1R structure (PDB: 5VAI) revealed the apo-state GLP-1R may adopt a similar extended ECD conformation as in the GLP-1-bound structure (Supplementary Fig

Researchers use Retatrutide in vitro to investigate receptor binding affinity and selectivity across its three targets, to measure cAMP-mediated downstream signalling, to perform comparative agonist potency profiling against dual-receptor controls, and to study receptor trafficking and ligand-competition dynamics in cell-based experimental formats