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An Ultimate 3000 UHPLC (Dionex) was coupled to the Q Exactive-Mass plus spectrometer (QE-MS, Thermo Scientific) for metabolite separation and detection

Recently, it has been demonstrated that glutaminolysis, which also supplies substrates for the Krebs cycle, facilitates the activation of OXPHOS and ATP production, thereby regulating efferocytosis efficiently through a non-canonical glutamine metabolism

The percentage of 5-mC was calculated using the second-order regression equation of the standard curve constructed with the negative and positive controls

All kinds of things can impact glutathione levels in dogs, from stress to medications and beyond