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The results from these trials are conflicting and thus difficult to interpret

Droplets were generated for each sample using the Automated Droplet Generator (BioRad), amplified for 50 cycles with primer/probe annealing at 56 C and extension for 1 min at 72 C, then analyzed on the QX200 droplet reader for FAM and HEX channels

Aging The older we get the more likely we are to be deficient in B-12.(3) The Institute of Medicine (IOM) recommends that adults over 50 supplement their diets with vitamin B-12