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Faecalibacterium prausnitzii is markedly reduced, and fecal butyrate concentration is correspondingly decreasedchanges that likely contribute to constipation development ( As an HDAC inhibitor, butyrate upregulates tight junction proteins (occludin, claudins, ZO-1) and the mucin MUC2, reinforcing intestinal barrier function ( At the CNS level, butyrate crosses the BBB and upregulates BDNF expression, promoting neuroplasticity

Figure 5: Representative chromatogram of LLOQC Sample Table 4: Intra and inter batch accuracy and precision