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The CY 2010 final rule adopted the proposal, with minor adjustments based on public comments, and explained that these minor adjustments were in part due to non-response bias that results when the characteristics of survey respondents differ in meaningful ways, such as in the mix of practices sizes, from the general population (74 FR 61749 through 61750)

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For wound healing and tissue repair, improvements can be observed within days to weeks, with most treatment protocols lasting 4-8 weeks for optimal results.

Both sterile water and saline lack any preservative