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Gene silencing Hepa-1c1c7 cells were transfected with small interfering RNA (siRNA) of carnitine palmitoyltransferase-1 (CPT-1), CPT-2, carnitine acylcarnitine translocase (CACT) and CrAT (Santa Cruz Biotechology, Santa Cruz, CA, USA) using LipofectamineRNAiMAX (Invitrogen, Waltham, MA, USA), and replaced with or without 800 M palmitic acid (PA) with Godex after 24 hours of transfection

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