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Am J Physiol Endocrinol Metab (2009) 296:E4739.10.1152/ajpendo.90636.2008 184 OvertonHABabbsAJDoelSMFyfeMCGardnerLSGriffinGet alDeorphanization of a G protein-coupled receptor for oleoylethanolamide and its use in the discovery of small-molecule hypophagic agents

Then, the fibroblasts were washed twice with Seahorse DMEM (SM media) supplemented with 1 mM pyruvate, 2 mM glutamine and 10 mM glucose (pH = 7.4) and equilibrated in a non-CO 2 incubator for 1 h