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There are two important groups of ligaments that hold the bones of the knee joint together, collateral and cruciate ligaments - medial collateral ligament (MCL) and lateral collateral ligament (LCL), and anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL)

Most protocols dose by injection close to the site of injury (subcutaneous), though no human pharmacokinetic standard exists

Although the use of HDX-NMR dates back to the 1970s and 1980s, the corresponding studies were primarily focused on the analysis of ordered proteins and their folding and interactions

The commonly used method is high-performance liquid chromatography, supplemented by other validation methods, as detailed below