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These organoids were treated with inflammatory stimuli (TNF, IFN, LPS), microbial structures (MDP, LPS), and an OXPHOS inhibitor (oligomycin) to assess the effects on GLP-1 secretion, EEC marker gene expression ( CHGA , TRIB3 ), and overall epithelial differentiation

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Wound healing was assessed using the Incucyte Live Cell Analysis System and quantified using Image J (version 2.0)