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For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)

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The hybridization buffer contains: 5xSSC, 50% deionized formamide, 100 g/ml autoclaved Herring sperm DNA, 50 g/ml Heparin and 0.1% Tween-20