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Cell culture Clonal insulin-secreting BRIN-BD11 cells were maintained in RPMI 1640 medium containing 11.1 mM glucose, supplemented with 10% foetal bovine serum (FBS), 100 U/ml penicillin and 0.1 mg/ml streptomycin, pH 7.4, as described previously 45

The greater efficacy of combination therapy is probably determined by the enhancement of NO synthase activity caused by the chronic administration of tadalafil 5 mg in the presence of a greater concentration of a NO source for the concomitant administration of a sufficient dosage of L-arginine

Gillingham MB, et al

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