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10.1111/tid.13312 154 LiJ.DuanN.SongS.NieD.YuM.WangJ.et al (2021)

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Losing the glutamate at position 3 sharply reduces its binding to IGF-binding proteins, which is why in cell culture it is reported to be roughly ten times more potent than intact IGF-1 at driving cell proliferation and hypertrophy [1]