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This is sometimes referred to as a methyl trap, where folate becomes functionally unavailable without sufficient B12, and vice versa

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First, a test solution can be prepared from a lyophilized or other solid form without introducing random ions or buffers if the product does not contain them

Its deficiency is manifested through changes in the number and morphology of erythrocytes, leucocytes and platelets, and by neurological alterations owed to the progressive demineralisation of the nervous system (a consequence of defective myelin synthesis)