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Recent studies have shown that GLP-1RAs therapy has excellent therapeutic effects in improving hyperandrogenaemia, increasing menstrual frequency, reducing manifestations of metabolic disorders such as obesity and IR, and reducing long-term cardiovascular risk in obese women with PCOS [164, 166]

Insulin-like growth factor long arginine 3 (IGF-1 LR3), is a widely used synthetic analog of IGF-1 with an arginine replacing glutamic acid at position 3 (arginine 3, R3), and an additional 13 amino acids at its N-terminus (long, L)

The 0.4 ml of supernatant was added to 0.5 ml of 1.25 M NaH 2 PO 4 and 0.1 ml of 100 mM NEM dissolved in 125 mM NaK phosphate buffer (pH 7.5)