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The relative integrated density of the samples compared to saline is used to compute the intensity ( Enzyme-linked immunosorbent assay TNF-, PGE 2, and NFB expressions were measured using the Rat TNF- ELISA kit (catalog no: E-EL-R0019), Rat PGE 2 ELISA kit (catalog no: E-EL-0034), and Rat NFB ELISA kit (catalog no: E-EL-R0674), respectively, in accordance with the manufacturers guidelines (Elabscience)

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