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doi: 10.1038/nrd.2017.162 33 KhomichO

GST Pull-Down assays For the GST pull-down assay, dilutions of the sonicated whole cell extract from GST only control, GST-Taf4, GST-Ada1, GST-HFDTaf4, GST-HFDAda1, Taf12LFLAG 3 and Taf12-FLAG 3 bearing strains were run on an SDS-PAGE gel along with marker dilutions to estimate the amount of fusion protein that was expressed in the lysate