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Jackson Immunoresearch, 1:400) in PTwH (100 PBS 10, 2 ml Tween 20, 1 ml of 10 mg ml 1 heparin stock solution) and 3% donkey serum at 37 C for 7 days, followed by washes in PTwH (100 PBS 10, 2 ml Tween 20, 1 ml of 10 mg ml 1 heparin stock solution): 1 10 min, 1 20 min, 1 30 min, 1 1 h, 1 2 h and 1 3 days

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At the same time, actuaries face considerable uncertainty in projecting future costs, due in part to evolving utilization patterns and the complex dynamics of pharmaceutical pricing