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Beyond direct genotoxic effects, heat stress has also been shown to significantly alter sperm epigenetic profiles, including miRNA expression patterns ( 3 Metabolic characteristics and functional basis of L-citrulline 3.1 The metabolic pivotal role of the arginine-NO pathway L-citrulline serves as a pivotal intermediate in the arginineNO metabolic pathway, functioning as a direct precursor for L-arginine

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Blood was collected from the retro-orbital sinus and placed into sterile tubes containing 1 mm EDTA, after which the mice were euthanized

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