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People with a deficiency often experience fatigue and weakness

It is also particularly valuable for patients who may have issues with their digestive system that may make other forms of increasing their B12 levels, such as pills and food, less effective

In a 2020 case study, researchers reported a young woman treated with multiple daily doses of 1 milligram (mg) of cyanocobalamin (synthetic vitamin B12) for severe pernicious anemia (lack of red blood cells due to vitamin B12 deficiency)

The proteins were embedded in a box of OPC water molecules (minimum distance of 12 from the solute to the boundaries of the box) and the net charge of the system was set to zero by neutralization with Cl-ions using the program tLEaP included in AmberTools21 ( 60