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Protein concentrations were determined using a BCA protein assay (Thermo Fisher Scientific) and 60 g of protein/lane were separated by SDSPAGE using e-PAGEL (ATTO Co., Tokyo, Japan) and were electro transferred onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories Inc., Hercules, CA) for 30 min at 25 V

PlexusDx Precision Peptide Genetic Test identifies variants in GLP1R, GIPR, FTO, and MC4R genes that influence how your body responds to GLP-1 therapy

was supported by the research grant (PD 138930) of the National Research, Development and Innovation Office, Budapest, Hungary, the Gedeon Richter Plc

Red flag symptoms requiring urgent GP consultation include abnormal vaginal bleeding, severe vulval pain or fissuring, pelvic pain, fever, spreading cellulitis, severe balanitis, difficulty retracting the foreskin with pain and swelling (possible paraphimosis), or symptoms that do not resolve with standard antifungal treatment