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Quantitative PCR was performed in an LC480 II Lightcycler (Roche) and using gene specific primers and Sybr Fast 2x Universal Master mix (Kapa biosystems, KK4611)

Instead, the Walsh protocol is used to measure whole blood histamine and plasma methylation levels

Symptoms of dementia that may be treated include but are not limited to: memory loss, difficulty communicating, difficulty with complex tasks, difficulty with planning and organizing, difficulty with coordination and motor functions, problems with disorientation (such as getting lost), personality changes, inability to reason, inappropriate behavior, paranoia, agitation, hallucinations, etc

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