glp 1 release Mechanisms of Action and Therapeutic Application of Glucagon-like Peptide-1 File:GLP-1 Signaling in the Body.jpg

Experimental groups consisted of 16 adult zebrafish and 20 larvae zebrafish

Targeted nanoformulation is a very efficient and attractive platform that might solve the issues of low solubility and metabolic stability of some therapeutic agents

Instead of targeting GLP-1 or GIP, eloralintide activates the amylin pathway, a separate hormone system involved in fullness and blood sugar control

For immunostaining, cells were permeabilized for 1 min using ice-cold PBS supplemented with 0.1% Triton X-100, blocked for 30 min with PBS supplemented with 1% BSA, incubated with the primary antibody in blocking buffer for 2 h at room temperature (RT), washed and subsequently incubated with secondary antibodies and DAPI in blocking buffer for 2 h at RT