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however, compounds holding esters 21A E seem to be retaining inhibitory activities, among which ( 21D ) were found to hold ester at 3-position with an IC 50 of 15 nM whereas, ( 21E ) with IC 50 of 20.2 nM holding ester at 4-position but 21C seems to be lost the inhibition activity and the best compounds amongst their class in regard showing similar inhibition potency, collectively both 3-substituted ester & benzoic acid coupled with 2-butnyl were best potent compounds & they selected for further evaluation of SAR

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