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GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating

In contrast, guanidinoacetate concentrations in the proximal intestine were elevated ( P 0.05) by dietary supplementation with either 1% or 2% glycine

10.3233/JAD-190662 J