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[DOI] [PMC free article] [PubMed] [Google Scholar] 271.Rubino F, Cummings DE, Eckel RH, et al
Additional immunostains for melanoma were requested on her bone marrow aspiration/biopsy sample because of the atypical disease course and the lack of previous history of superficial melanoma
After lysis and release of the solubilized protein, the GST tagged protein is purified by affinity chromatography by adding 0.5ml glutathione resin to the clarified lysate
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