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And so very early on as soon as I came out of pharmacy school went into a private practice and started doing the research on glutathione immediately and it didn't take me too long maybe like three or four years into my career and my first project I landed was to work on glutathione

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Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)