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While previous fiber photometry results showed that administration of GLP-1 or liraglutide did not significantly alter ARC AgRP/NPY neuron calcium signals post 30-minute injection, after 2 hours post-injection, the rapid suppression of AgRP calcium signals after cognition of food was indeed attenuated by liraglutide [49,82,85]

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After washing, cells were seeded on collagen-coated plates and cultured in attachment medium in 6-well plates under humidity conditions in 95% air and 5% CO 2 at 37 C for 24 h before treatment