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Monocyte chemoattractant protein-1-induced tissue inflammation is critical for the development of renal injury but not type 2 diabetes in obese db/db mice

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To test this, we took advantage of the reversible nature of disulfide bond formation and incubated the 8 modified cysteine-SpyCatcher003 mutants with 10 mM TCEP (tris(2-carboxyethyl)phosphine), a common reducing agent