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Recruitment for ME/CFS patients started just before COVID related restrictions were introduced and research was suspended: one patient and one healthy control were recruited in February 2020, others from February 2021

It was first isolated and described by Monnier and colleagues in 1977 from the thalamic venous blood of rabbits during electrically induced slow-wave sleep

After exposure to an isotonic environment through the addition of NaCl, the system was made electrically stable by adding counter ions, a NoseHoover thermostat maintaining a 300-k temperature, and pressure of 1.01325 bars by MartynaTobiasKlien barostate with 100 ns as the time of the simulations