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TLR4 activation was assessed in the HEK293 cells, while the activation of NF-B p65, NLRP3, and Keap1/Nrf2, as well as the release of the pro-inflammatory cytokines IL-1 and TNF-, were evaluated in THP-1 macrophages through western blotting, RT-PCR, and ELISA techniques

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The most common dosages 31 used in studies 32 range from 2000-4000 mg of myo-inositol and 50-100 mg of d-chiro-inositol a day to match the physiological ratio that exists in the body (40 to 1 of MI to DCI 33 )

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