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Step 5: Dispose of the needle safely Place the used needle (or the entire single-use pen) into an approved sharps container

Then liposomes labeled with the lipophilic fluorescent dye DiD were added to the apical chamber of the Transwell plates and incubated at 37 C for 2 h, after which the bEnd.3 cells were fixed with 4% PFA at room temperature for 1 h and stained with DAPI at room temperature for 5 min