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Transcript abundance also increased in infested resistant plants when the unique primer pair was used for qPCR analysis even though the variation was bigger than that when PCR was carried with the primer pair common to three different sequences among different replicates (Figure 2c)

The in vitro analyses of the IgE recognition were performed by RAST inhibition assay using sera with RAST values to AXO-poly-L-lysine (AXO-PLL) higher than 7%