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The IV fluid contained in this drip is normal saline (physiological saline), which is composed of water and electrolytes (sodium and chloride)

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Subsequently, the sections were incubated with secondary antibody (goat anti-rabbit), rinsed with phosphate buffer saline (PBS), stained with DAB, and hematoxylin and eosin (H&E) staining, dehydrated, transparentized, and mounted before microscopic analysis